CleavageSitesTableforFusionProteins：PROTEASECOMPANYProteaseCaptureThrombinAmersham-Biosciences,Benzamidine-AgaroseLeu-Val-Pro-Arg��Gly-SerNovagen,SIGMA,RocheFactorXaAmersham-Biosciences,NewBenzamidine-AgaroseIle-Glu/Asp-Gly-Arg��EnglandBiolabs,RocheEnterokinaseNewEnglandBiolabs,TrypsinInhibitor-Asp-Asp-Asp-Asp-Lys��Novagen,RocheAgaroseTEVproteaseInvitrogen–LifeNi-NTA(6Hisrecomb.Glu-Asn-Leu-Tyr-Phe-Gln��GlyTechnologiesTEV)PreScissionAmersham-BiosciencesGSTrapforGSTfusionLeu-Glu-Val-Leu-Phe-Gln��Gly-ProenzymeTAGZymeQiagenNi-NTA(6Hisrecomb.His-tagremovalbyExoproteolyticDigestionenzyme)InteinSiteNewEnglandBiolabsDTTeliminationbydithiothreitolcleavagedialysisHRV3CProteaseNovagenNi-NTA(6Hisrecomb.Leu-Glu-Val-Leu-Phe-Gln��Gly-Proenzyme)SUMOProteaseInvitrogen–LifeNi-NTA(6Hisrecomb.recognizethetertiarystructureoftheTechnologies,LifeSensorsenzyme)ubiquitin-like(UBL)protein,SUMO内容总结（1）CleavageSitesTableforFusionProteins：
